Composite

Part:BBa_K2374005:Design

Designed by: Jianfeng Zhou   Group: iGEM17_Tongji_China   (2017-10-26)


TH-GAL4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 137
    Illegal XhoI site found at 676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 595
    Illegal BsaI.rc site found at 2271
    Illegal SapI site found at 960


Design Notes

We use seamless cloning to make it.


Source

TH promoter -> Drosophila melanogaster GAL4 -> Saccharomyces cerevisiae

References